High sensitivity is one of the key advantages of the ELISA kit, and this is closely related to the sample being tested. In general, if the concentration of the target protein in the sample is relatively high, the need for high sensitivity is less. However, when the concentration of the target protein is low, sensitivity becomes a critical factor that must be taken into account. Typically, the minimum concentration on the standard curve of the kit is considered reliable. Below this value, due to the S-shaped nature of the curve, results may become less accurate and more of an estimate. Combined with experimental variability, this could lead to inconsistent outcomes. Therefore, it's recommended to focus on the lowest detectable concentration on the standard curve rather than the sensitivity value listed on the kit itself. So what is the typical detection range of an ELISA kit? Most diagnostic reagents are designed to detect proteins in the range of ng/mL. However, the actual concentration of the target protein in a sample can vary significantly depending on the type of sample used. Before conducting an experiment, it's important to check literature and perform preliminary tests to determine whether the sample falls within the detection range of the purchased kit. If not, there are two common approaches: A. If the target protein concentration is too low, consider using a more sensitive kit or concentrating the sample before testing. We take pride in offering high-quality ELISA kits, antibodies, and biological reagents. Our products are consistently praised by researchers for their reliability, competitive pricing, fast delivery, and exceptional customer service. We are dedicated to providing comprehensive solutions for scientific research, ensuring that every user receives the best possible support. From product selection to technical guidance, we are here to help you achieve accurate and consistent results in your experiments. class d amplifier board 200 watt,mono amplifier board,class ab amplifier board,subwoofer plate amplifier Guangzhou Aiwo Audio Technology Co., LTD , https://www.aiwoaudio.com
B. If the concentration is too high, dilute the sample appropriately before proceeding with the assay.
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